TABLE 1.
N2-fixing activities of single cultures and cocultures of clostridial isolates with accompanying bacteria in semisolid RMR medium
| Phylogenetic groupb | Clostridial isolate | ARA (nmol of ethylene produced h−1 tube−1)a
|
||
|---|---|---|---|---|
| Single culturec
|
Coculture with aerobic, nondiazotrophic isolate in aird | |||
| Air | Anoxic | |||
| I | U201 | ND | ++ | + (U221) |
| U101 | ND | ++ | +++ (U111) | |
| B907-1 | ND | + | +++ (B907-2) | |
| B556-1 | ND | ++ | +++ (B556-2), ND (B556-3) | |
| Kas401-4 | ND | ++ | +++ (Kas403-3), +++ (Kas401-2), +++ (Kas401-1), ++ (Kas403-2) | |
| Sukash-1 | ND | ++ | ++ (Sukash-2) | |
| II | Kas107-2 | ND | ND* | ++ (Kas107-3), + (Kas103-2), ND (Kas105-5), ND (Kas105-6), ND (Kas107-4) |
| OkiF101 | ND | ND* | ++ (OkiF105), ND (OkiF102), ND (OKiF103), ND (OKiF104) | |
| OkiN108 | ND | ND* | ++ (OkiN104), ++ (OkiN106), ++ (OkiN102), ++ (OkiN105), ++ (OKiN103), ND (OKiN107) | |
| III | B904-4 | ND | ++ | ++ (B904-3), ++ (B904-1), ++ (B904-2) |
| IV | Kas107-1 | ++ | +++ | +++ (Kas105-5), ++ (Kas107-4), ++ (Kas107-3), ++ (Kas105-6), + (Kas103-2) |
| UsIt102-1 | + | ++ | ++ (UsuIR-1), ++ (UsuIR-2) | |
| Kas301-1 | ++ | ++ | +++ (Kas302) | |
| Kas203-1 | ND | +++ | ++ (Kas203-3), ++ (Kas203-4), + (Kas203-5) | |
| Kas104-4 | + | ++ | +++ (Kas102-2), + (Kas104-5) | |
| Kas401-3 | + | +++ | ++ (Kas403-3), ++ (Kas401-2), + (Kas401-1), +(Kas403-2) | |
| Kas404-1 | + | ++ | ++ (Kas404-3), ++ (Kas404-4), ++ (Kas404-2), ND (Kas402-1), ND (Kas402-2) | |
| B910-1 | ND | ++ | ++ (B910-5), ++ (B910-4M), ++ (B910-3T), ++ (B910-3W), ++ (B910-4S) | |
| P303 | ND | ++ | +++ (P312), ++ (P311) | |
| P301 | ND | ++ | +++ (P312), +++ (P311) | |
| V | B915-1 | ND | +++ | +++ (B915-2) |
| Kas202-1 | ND | ++ | +++ (Kas202-3), ++ (Kas202-2), ++ (Kas202-4), + (Kas202-5) | |
| UsIt102-2 | ND | +++ | +++ (UsuIR-1), +++ (UsuIR-2) | |
| UsIt101-1 | ND | +++ | +++ (UsuIS-1), +++ (UsuIS-2) | |
| UsS102-1 | ND | +++ | +++ (UsuSR-2), +++ (UsuSR-1) | |
| UsS101-2 | ND | +++ | +++ (UsuSS-2), +++ (UsuSS-1) | |
| B908-1 | ND | +++ | +++ (B908-2), ++ (B908-3) | |
| B913-1 | ND | +++ | +++ (B913-2) | |
| Kas201-1 | ND | +++ | +++ (Kas201-4), ND (Kas201-3) | |
| B906-1 | ND | ++ | +++ (B906-2) | |
| UsIt102-3 | ND | ++ | +++ (UsuIR-1), +++ (UsuIR-2) | |
| Kas106-4 | ND | ++ | ++ (Kas101-2), +++ (Kas106-5) | |
| Kas402-3 | ND | ++ | ++ (Kas404-3), ++ (Kas404-2), ++ (Kas404-4), + (Kas402-1), ND (Kas402-2) | |
| UsS101-1 | ND | +++ | +++ (UsuSS-1), +++ (UsuSS-2) | |
| B902-1 | ND | ++ | +++ (B902-2) | |
| B901-1 | ND | ++ | +++ (B901-2) | |
a
+, ++, and +++, 0.2 to 2, 2 to 20, and 20 to 200 nmol h−1 tube−1, respectively. ND, not detected (<0.1 nmol h−1 tube−1).
b
Phylogenetic groups were defined as shown in Fig. 3 according to 16S ribosomal DNA sequences.
c
ARA of the respective clostridial isolate in semisolid RMR medium in air and under anoxic conditions in an AnaeroPack system (Mitsubishi, Tokyo, Japan). Asterisk, no ARA under anoxic conditions but positive ARA when cocultured with the appropriate accompanying bacteria.
d
Isolates in the same row originated from identical plant materials.