TABLE 2.
N2-fixing activity of clostridial isolates cocultured with accompanying bacteria of different origins in semisolid RMR medium
| Culture condition | Acompanying bacterium | ARA (nmol of ethylene produced h−1 tube−1) of clostridial isolatea
|
||
|---|---|---|---|---|
| Kas107-2 | OkiF101 | OkiN108 | ||
| Cocultures | ||||
| Air | Kas107-3 | 15.1 ± 1.1* | 18.4 ± 1.1 | 24.1 ± 1.5 |
| Kas107-4 | ND | ND | ND | |
| OkiF105 | 9.0 ± 1.1 | 13.3 ± 2.2* | 14.7 ± 0.5 | |
| OkiF102 | ND | ND | ND | |
| OkiN104 | 5.9 ± 0.2 | 7.4 ± 0.9 | 6.7 ± 0.5* | |
| OkiN107 | ND | ND | ND | |
| Single cultures | ||||
| Air | None | ND | ND | ND |
| Anoxic | None | ND | ND | ND |
a
ND, not detected (<0.3 nmol of ethylene h−1 tube−1). ARAs of clostridial isolates (group II) in semisolid RMR medium in air and under anoxic conditions in an AnaeroPack system (Mitsubishi) are shown. Bacteria with Kas107, OkiF, and OkiN prefixes were isolated from stems of M. sinensis and Saccharum hybrid sp. (sugarcane cultivars F177 and Ni9), respectively. Asterisks, original combinations of ANFICOs. Values are means with standard deviations for triplicate determinations.