TABLE 1.
Hydrodynamic and microbiological characteristics of original soil, soil from control columns, and soil from columns amended with one, two, or three pulses of 2,4-D
Treatment | Soila | Apparent dispersion coefficient (cm2 h−1) | Solute exchange coefficient (h−1) | Fraction of mobile water | No. of heterotrophs per g [dry wt] of soil | No. of 2,4-D degraders per g [dry wt] of soil | % of 2,4-D degraded | Determination of spatial distributionb |
---|---|---|---|---|---|---|---|---|
Original soil | S | NRc | NR | NR | 3.6 × 107 | 1.9 × 102 | NR | + |
Control column | Ca | NDd | ND | ND | 2.7 × 107 | 3.1 × 102 | + | |
Cb | ND | ND | ND | 4.0 × 107 | 3.7 × 103 | + | ||
Cc | ND | ND | ND | ND | ND | NR | + | |
Cd | ND | ND | ND | ND | ND | + | ||
Ce | ND | ND | ND | ND | ND | + | ||
Column amended | IP1a | 0.05 | 0.03 | 0.83 | 3.8 × 107 | 2.0 × 105 | 25 | + |
with one pulse | IP1b | ND | ND | ND | ND | 5.6 × 105 | ND | + |
IP1c | ND | ND | ND | ND | ND | ND | + | |
Column amended | IIP1a | 0.05 | 0.03 | 0.83 | ND | ND | 46 | − |
with two pulses | IIP2a | 0.22 | 0.03 | 0.82 | 5.7 × 107 | 5.5 × 106 | 97 | + |
IIP2b | ND | ND | ND | 1.4 × 107 | 9.4 × 105 | 75 | + | |
IIP2c | ND | ND | ND | 3.8 × 107 | 1.1 × 106 | 98 | + | |
Column amended | IIIP1 | 0.05 | 0.03 | 0.83 | ND | ND | 50 | − |
with three pulses | IIIP2 | 0.22 | 0.03 | 0.83 | ND | ND | 59 | − |
IIIP3 | 0.22 | 0.03 | 0.83 | 3.8 × 107 | 6.2 × 106 | 73 | + |
S, original soil; C, soil from control column; IP, IIP, and IIIP, soil from columns amended with one, two, and three pulses of 2,4-D, respectively. The first, second, and third pulses are indicated by 1, 2, and 3, respectively. The suffixes a, b, c, d, and e indicate replicates.
+, spatial microsampling performed; −, spatial microsampling not performed.
NR, not relevant.
ND, not determined.