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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Pediatr Surg. 2014 Jan 31;49(6):938–944. doi: 10.1016/j.jpedsurg.2014.01.030

Figure 1. Transfection efficiency of human HB-EGF in MSC.

Figure 1

(A) EGFP-positive BM-MSC were co-transfected with RFP and either human HB-EGF or scrambled control plasmids. RFP positive cells (red) were visualized under fluorescence microscopy. Similar results were found for AF-MSC (not shown). Total RNA was extracted from non-transfected, scrambled control plasmid transfected, or HB-EGF transfected BM-MSC (B) or AF-MSC (C) and real-time RT-PCR performed. Data were normalized to GAPDH and are shown relative to the expression of the HB-EGF gene (*p<0.05 vs. non-transfected or scramble transfected controls).