TABLE 2.

Quantitative PCR demonstrating transformation of plant tissue as well as persistence of bacteria^a

DNA extract (dilution)	Mean Ct value from PCRb of:
	COX	fliG	rol
Root (undiluted)	24.64	21.32	18.50
NCPPB 4042 (undiluted)	—c	16.06	17.19
NCPPB 4042 (10−1)	—	19.30	20.72
NCPPB 4042 (10−2)	—	22.89	24.20
NCPPB 4042 (10−3)	—	25.97	27.52

^aC_t values are from three separate TaqMan assays performed on a root DNA extract obtained from a cucumber cotyledon inoculated with NCPPB 4042 and a dilution series obtained from a pure culture of NCPPB 4042.

^bFor information on procedures for COX PCR, see Weller et al. (34); for fliG PCR, see Weller et al. (36); for rol PCR, see Weller and Stead (35).

^c—, no fluorescence above threshold was detected.