TABLE 1.
Relative abundance of methanotrophic groups based on frequencies of pmoA genes in clone librariesa
| Phylogenetic group | Clone group | No. of clones | Relative abundance (%) | Next relative (sequence identity) |
|---|---|---|---|---|
| Type I | B1 | 22 | 29 | Methylobacter sp. strain LW12 (3) (93-97%) |
| B2 | 5 | 7 | Clones FW-36 and FW-50 (32) and pAMC512 (11) (94-99%) | |
| B3 | 9 | 13 | Clone Fw-18 (32) (98-100%) | |
| B4 | 1 | 1 | Clone Zhenjiang 5 (GenBank accession no. AJ430816) (94%) | |
| B5 | 1 | 1 | Clone pmoA-RC4 (21) (94%) | |
| B6 | 10 | 13 | Clone N-368 (GenBank accession no. AJ317928) (88-94%) | |
| Type II | B7 | 27 | 36 | Methylocystis spp. (13, 22) (96-99%), Methylosinus sp. strain LW2 (11) (95-99%) |
a
Clone groups B1 to B7 contain the sequences obtained in this study and are based on at least 90% sequence identity within one group. The sequences of the clones are compared to their next phylogenetic relatives.