FIG. 2.
Northern blot analysis and FB1 production of the wild type cultured on separated maize kernel germ and degermed tissues. Total RNA (10 μg) isolated from the wild-type strain was cultured for 7 days on maize kernel germ (lane 1) and degermed (lane 2) fractions. The blot was probed with ZFR1 (A) and TUB2 (B) as a loading control. (C) Quantification of FB1 as determined by HPLC extracted from germ (G) and degermed (DG) kernel fractions. Averages of three repetitions are shown with error bars showing standard errors.