FIG. 5.

Northern blot analysis of wild-type and ZFR1 overexpression strains. Total RNAs (10 μg) isolated from the zfr1Δ mutant (zfr1 deletion strain, lane 1), the wild type (lane 2), the fcc1Δ/GPD::ZFR1 mutant (fcc1Δ mutant strain transformed with a ZFR1 overexpression construct [GPD::ZFR1], lane 3), the zfr1Δ GPD::ZFR1 mutant (zfr1 deletion strain transformed with GPD::ZFR1, lane 4), and the fcc1Δ mutant (fcc1 deletion strain) extracted from cultures grown for 7 days on whole cracked maize kernels were separated by electrophoresis in a 1.2% agarose-formaldehyde gel, transferred to a nylon membrane, and hybridized with ³²P-labeled FUM1- and FUM8-specific probes (top), a ³²P-labeled ZFR1-specific probe (middle), and a ³²P-labeled β-tubulin-specific probe (bottom).