Effect of ZFR1 overexpression on FB1 production. The wild-type, zfr1Δ mutant (zfr1 deletion mutant), zfr1Δ-R mutant (zfr1Δ mutant transformed with ZFR1), zfr1Δ GPD::ZFR1 mutant (zfr1Δ mutant strain transformed with a GPD::ZFR1 overexpression construct), fcc1Δ mutant, and fcc1Δ GPD::ZFR1 mutant (fcc1Δ mutant transformed with a GPD::ZFR1 overexpression construct) strains were cultured for 7 days on whole cracked maize kernels and analyzed for FB1 production. All values are averages of three repetitions, and error bars indicate standard errors. n.d. = none detected.