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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Virology. 2014 Apr 15;0:205–219. doi: 10.1016/j.virol.2014.03.010

Figure 1.

Figure 1

HeLa cells infected with the E1B-55K/E4orf3 double-mutant virus are heterogeneous in appearance at late times of infection. (A) HeLa cells were mock-infected or infected at an MOI of 10 with the E1B-55K-deleted virus (ΔE1B-55K) or E1B-55K/E4orf3 double-mutant virus (ΔE1B-55K/ΔE4orf3). Representative images captured by phase-contrast microscopy at 72 hpi are shown. (B) HeLa cells were infected with the indicated viruses and the apparent diameters of detached cells were determined as described in the Materials and Methods. The mean diameters of 120 to 250 cells are summarized in the box and whiskers plot. Differences among mean cell diameters from the three independent experiments were significant (p = 0.008, one-way ANOVA allowing for unequal variances among groups.) Application of the t-test with correction for multiple comparisons indicated that cells infected with the E1B-55K/E4orf3 double-mutant virus were significantly larger than other virus-infected cells (p < 0.01). (C) HeLa cells were infected at an MOI of 10 with the indicated viruses and live cells were analyzed for size (Forward Scatter) and intracellular or surface perturbations (Side Scatter) by flow cytometry. The percentage of cells found in the upper left quadrant is indicated in each panel. (D) HeLa cells were mock-infected or infected with wild-type or double-mutant viruses at an MOI of 10 and processed for scanning electron microscopy at 72 hpi.