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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Virology. 2014 Apr 15;0:205–219. doi: 10.1016/j.virol.2014.03.010

Figure 6.

Figure 6

PAR levels increase in adenovirus-infected cells and PARP-1 activity is necessary for nuclear fragmentation in double-mutant virus-infected cells. HeLa cells were infected with the viruses indicated at an MOI of 10. (A) Cells were fixed and stained by indirect immunofluorescence for PAR. The fluorescence intensity of between 100 and 1000 individual cells for each virus was determined by quantitative immunofluorescence microscopy. The log of the total fluorescence intensity is plotted as a density distribution. (B) The mean fluorescence intensity of PAR-stained cells from three independent experiments are plotted with open symbols. The geometric mean for the three experiments is indicated with the horizontal bar. (C) Infected cells were treated with PARP-1-inhibitor 3-aminobenzamide. Cells were stained for DNA at 72 hpi and the fraction of cells containing fragmented nuclei was determined. Results from a representative experiment of three independent experiments are shown. Error bars indicate the upper limit of the 95% exact binomial confidence interval for the representative experiment.