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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Virology. 2014 Apr 10;0:171–178. doi: 10.1016/j.virol.2014.03.022

Table 1. Complementation of gene 4 and gene 5 amber mutant phage by deletion plasmids.

The complementation efficiency of the delta-domain deletion-mutant plasmids was compared to complementation of the wild-type expression plasmid using spot test scores to calculate a relative efficiency of plating (EOP), as described under Materials and Methods. The delta-domain deletion-plasmids were all reduced by 3 to 4 log10 in complementation efficiency in comparison to wild type.

Plasmid Wild-type relative EOP Gene 5 amber relative EOP Gene 4 amber relative EOP
Wild-type 1* 1* 1*
pNoDelta2 1 0.0001 n/a
Del1 1 0.001 1
Del2 1 0.001 1
Del3 1 0.001 1
Del4 1 0.001 1

The asterisks (*) indicate that the wild-type plasmid scores were set to 1 and used as the denominator in EOP calculations. (n/a: not applicable)