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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Virology. 2014 Apr 5;0:157–170. doi: 10.1016/j.virol.2014.03.016

Figure 4.

Figure 4

Composition of particles (primarily heads) fractionated by rate zonal centrifugation in a sucrose gradient. (a) Low-field, low concentration agarose gel electrophoresis, with GelStar staining, performed with DNA expelled from capsids. (b) SDSPAGE of the proteins, with Sypro Ruby staining. The growth medium had 0.1 M NaCl. The arrowheads indicate the origins of electrophoresis; the arrow indicates the direction of electrophoresis. Symbols above the lanes for (a): CH, conventional head peak; MH, modified head peak; ϕ-T3, DNA of wild type T3 phage; ϕ-λ and ϕ-T7, DNAs of wild type phages λ and T7; B, restriction endonuclease Bgl II digest of phage T7 mature DNA; H, restriction endonuclease Hind III digest of phage λ mature DNA; X, restriction endonuclease Xba I digest of phage T7 mature DNA. The numbers above lanes in (a) are fraction numbers from a sucrose gradient like the one in Figure 3b. Symbols above the lanes for (b), when not the same as in (a): ϕ-WT, proteins of wild type phage T3; ϕ-SR, proteins of T3SR3-1 phage; CI, proteins of wild type T3 capsid I. The numbers at the right provide the encoding gene (Pajunen et al., 2002) for the protein indicated.