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. 2014 Jun;184(6):1831–1842. doi: 10.1016/j.ajpath.2014.03.003

Figure 6.

Figure 6

Akt pathway activation in VEH- and ActRIIB-treated mice. A: Western blot analysis shows the relative expression of Akt, p-Akt (at Ser473 and Thr308), total P70/S6K, p-P70/S6K (at Thr421/Ser424), rpS6, p-rpS6 (at Ser240/244), p-4EBP1 (at Thr37/46), and p-eEF2K (at Ser366) in the quadriceps and gastrocnemius muscles of VEH- and ActRIIB-treated WT and R69C mice at 6 MOL. GAPDH staining is used as a basis for loading and quantification studies. The numbers under each set of two lanes refer to individual mice from the study. B: Quantification of the Western blot analysis results shown in A. Expression is normalized to the GAPDH expression for each lane. These values were obtained from gels in which the WT and R69C samples were run in parallel on the same gels and immunoblotted on the same membranes. Data are expressed as means ± SEM. ∗P < 0.05. ActRIIB, ActRIIB-mFC; G, gastrocnemius; Q, quadriceps; R69C, Mtm1 p.R69C; VEH, vehicle.