Figure 3.
Mice with RB-sufficient and RB-deficient livers were treated with TCPOBOP for 72 hours. A: Liver extracts were prepared and immunoblotted for the indicated proteins; all samples were analyzed in duplicate. B: Mice with RB-sufficient and RB-deficient livers were treated with TCPOBOP, DEN, or CCl4 and sacrificed at the indicated time points. Liver extracts were prepared and immunoblotted for the indicated proteins; all samples were analyzed in duplicate. C: Hepatocyte ploidy was measured by flow cytometry. D: Mitosis measured by phospho-histone H3 staining. Data are from four mice in each group and represent means ± SD (C and D).