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. 2014 Jun;184(6):1877–1889. doi: 10.1016/j.ajpath.2014.02.010

Figure 2.

Figure 2

Time course of expression of P-selectin and neutrophil markers after M. pulmonis infection. Whole mounts of mouse trachea stained for P-selectin (red) and endothelial cells (Pecam1, green). A: In a pathogen-free mouse, P-selectin immunoreactivity was faint or absent in capillaries (arrows), but was moderate in venules (arrowheads) located between cartilage rings. B: At day 7 after infection, P-selectin was moderate to strong in enlarged remodeled vessels (arrows) over cartilage. C: Enlargement of boxed region in B. Most P-selectin immunoreactivity was in granules in endothelial cells of remodeled vessels. Pecam1 staining at endothelial cell borders of remodeled vessel was stronger, wider, and more irregular than in capillaries (A). D–G: Time course of expression of P-selectin (D), the neutrophil marker S100a9 (E), Cxcr2 (F), and the severity of infection reflected by expression of M. pulmonis 16S rRNA (G) assessed by RT-qPCR. Black circles indicate values for individual mice and the lines show the group mean. N = 3 to 5 mice per group. ∗P < 0.05 compared with the pathogen-free group. Scale bars: 50 μm in (A and B); 10 μm (C).