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. 2014 Jun;184(6):1877–1889. doi: 10.1016/j.ajpath.2014.02.010

Figure 3.

Figure 3

Effect of neutrophil-depleting antibodies on neutrophil influx and vascular remodeling after M. pulmonis infection. Whole mounts of mouse trachea comparing the number of neutrophils (S100a8, green) (A–C) and size of blood vessels (Pecam1, red) (D–F) in mucosa over a cartilage ring in mice given control IgG (A and D), RB6-8C5 (B and E), or 1A8 (C and F) throughout the 7-day infection. Neutrophil influx and vascular enlargement after infection are much less after RB6-8C5 or 1A8 than after the control IgG. Measurements of neutrophils (G) and blood vessel diameter (H) in tracheal mucosa over cartilage rings on day 7 of infection after antibody treatment. N = 4 to 6 mice per group; ∗P < 0.05 compared with IgG group. I: Linear regression of blood vessel size versus number of neutrophils on day 7 of infection for all mice in the four treatment groups. N = 4 to 5 mice per group, P < 0.001. Capillary enlargement was correlated positively to the number of neutrophils and was not present in the absence of neutrophil influx in pathogen-free mice or in infected mice after neutrophil depletion. Scale bar = 50 μm (A–F).