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. 2014 Apr 9;165(2):528–533. doi: 10.1104/pp.113.231274

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Figure 3. — Deficient funicular guidance of mpk3 mpk6 pollen tubes in vivo. A, Double fluorescent reporter-tagged complementation line. Plants of mpk3^–^/^– mpk6^–^/^– gMPK6-YFP⁺^/^– TdTOM⁺^/⁺ genotype produce double mutant pollen tagged by TdTOM only and complemented pollen tagged by both YFP and TdTOM. B to D, Fluorescent images of TdTOM (B), YFP (C), and merged (D) channels of pollen grains from mpk3^–^/^– mpk6^–^/^– gMPK6-YFP⁺^/^– TdTOM⁺^/⁺ plants. Pollen of mpk3 mpk6 TdTOM genotype is indicated by the cyan color in merged channel. Pollen of mpk3 mpk6 gMPK6-YFP TdTOM genotype is indicated by the white color in merged channel. Bar = 25 µm. E to G, Pistil with carpel and ovules removed at 7 h after pollination. The double mutant and complemented pollen tubes showed similar growth rates and morphology in the transmission tract. E, TdTOM. F, YFP. G, Merged. Bar = 100 µm. H to J, Pistil with carpel removed at 7 h after pollination to exhibit the ovules. In this part of the pistil, all ovules were fertilized by complemented pollen tubes. Note no double mutant pollen tubes were observed on funiculus. H, TdTOM. I, YFP. J, Merged. Bar = 100 µm.