Figure 1.

Experimental design and data analysis. A, Design of the HS treatment for P. patens protonema. Arrows indicate the time of collecting samples for total RNA extraction. Two-week-old moss protonemal cells grown at 25°C were shifted to 38°C for 1 h (1st HS), recovered at 25°C for 5.5 h, and then shifted to 38°C for 1 h (2nd HS). Protonemal cells remaining at 25°C were collected as the control (C). B, Steps for preparing cDNA libraries and pipeline for RNA-seq data analysis. C, Distributions of RNA-seq reads from pooled (All) and control, 1st HS, and 2nd HS samples on the annotated P. patens genome.