Induced cell death in ZmHb-expressing domains regulates embryogenesis. A, Identification of TUNEL-positive cells in immature embryos (d 7, P medium) obtained from the PZmHb2:AtKOD lines, the PZmHb1:AtKOD lines, and the wild-type line. Bars = 20 μm. B, Number of mature embryos (d 21, D medium) produced by three PZmHb1:AtKOD lines and three PZmHb2:AtKOD lines. Numbers in parentheses indicate the KOD-actin ratio in the respective lines. Values are means ± ses of four biological replicates and expressed as a percentage of wild type (set at 100%). WT, Wild type. *, Statistically significant difference (P ≤ 0.05) from wild type.