. 2014 May 1;165(2):810–825. doi: 10.1104/pp.114.239335

Table I. Effects of the NO donor DEA/NO on the release of Zn²⁺ from the recombinant MBP5 and the MBP5-ZmMT4 fusion protein.

Medium was supplemented with 0.4 mm ZnSO₄. Values are means ± ses of three biological replicates. Zn²⁺ measurements were performed by the dithizone method (Paradkar and Williams, 1994).

Protein	Concentration	Zn²⁺ Released		Acidification	DEA/NO
Protein	Concentration	Acidification	DEA/NO	Acidification	DEA/NO
	mm			%
MBP5	4.15 ± 0.13	0.01 ± 0.01	0.01 ± 0.01
MBP5-ZmMT4	4.15 ± 0.21	0.76 ± 0.02	0.63 ± 0.05	100^a	83^a

The percentage of Zn²⁺ recovered after the DEA/NO treatment (Zn²⁺ released by NO) compared with the acidification assay (Zn²⁺ stored in MT4).

Table I. Effects of the NO donor DEA/NO on the release of Zn2+ from the recombinant MBP5 and the MBP5-ZmMT4 fusion protein.

Table I. Effects of the NO donor DEA/NO on the release of Zn²⁺ from the recombinant MBP5 and the MBP5-ZmMT4 fusion protein.