Table III. Kinetic constants of VvGT7a, VvGT7b, and VvGT7c determined for the substrates nerol, citronellol, geraniol, and UDP-Glc.
Allele | Substrate | Km | kcat | kcat/Km |
---|---|---|---|---|
mm | 10−3 s−1 | 10−3 s−1 mm−1 | ||
VvGT7a | Nerol | 0.417 ± 0.004 | 0.4 | 1.0 |
Citronellol | 0.433 ± 0.062 | 0.2 | 0.5 | |
Geraniol | 0.464 ± 0.056 | 0.1 | 0.2 | |
UDP-Glc | 0.052 ± 0.009 | 0.3 | 5.8 | |
VvGT7b | Nerol | 0.204 ± 0.027 | 2.0 | 9.8 |
Citronellol | 0.306 ± 0.075 | 0.2 | 0.7 | |
Geraniol | 0.396 ± 0.012 | 0.2 | 0.5 | |
UDP-Glc | 0.048 ± 0.001 | 1.2 | 25.0 | |
VvGT7c | Nerol | 0.211 ± 0.016 | 1.4 | 6.6 |
Citronellol | 0.445 ± 0.021 | 0.3 | 0.7 | |
Geraniol | 0.321 ± 0.002 | 0.3 | 0.9 | |
UDP-Glc | 0.056 ± 0.003 | 1.4 | 25.0 |