Table I. Physiological and biochemical characteristics of the CA mutants.
Net Rate of CO2 Assimilationa |
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---|---|---|---|---|---|---|---|---|
Sample | Quantum Yield | Low CO2 (9.3 Pa) | Ambient CO2 (37.2 Pa) | High CO2 (139.5 Pa) | Rubisco Activity | PEPC Activity | Total Leaf Nitrogen | Dry Biomass |
mol mol−1 | μmol m−2 s−1 | mg g−1 dry wt | g | |||||
ca1-m1 | ||||||||
Wild type | 0.027 ± 0.003 | 10.3 ± 0.5 | 27.5 ± 1.2 | 27.7 ± 0.5 | 38.9 ± 1.8 | 217.7 ± 30.6 | 52.5 ± 2.1 | 6.1 ± 0.6 |
Heterozygote | 0.027 ± 0.001 | 8.3 ± 0.5 | 23.0 ± 1.7 | 25.5 ± 0.3 | 39.8 ± 2.6 | 221.8 ± 29.8 | 50.9 ± 1.8 | 7.6 ± 0.6 |
Homozygote | 0.030 ± 0.003 | 8.7 ± 0.3 | 26.1 ± 0.6 | 29.8 ± 1.0 | 38.9 ± 3.8 | 212.8 ± 23.1 | 54.7 ± 4.3 | 6.8 ± 0.7 |
ca1-m2 | ||||||||
Wild type | 0.031 ± 0.001 | 9.6 ± 0.3 | 27.6 ± 0.8 | 29.5 ± 1.5 | 38.7 ± 5.2 | 176.0 ± 24.9 | 54.3 ± 1.7 | 7.7 ± 0.2 |
Heterozygote | 0.034 ± 0.001 | 9.0 ± 0.1 | 26.8 ± 0.6 | 30.1 ± 1.9 | 44.7 ± 4.3 | 196.9 ± 27.2 | 52.0 ± 2.5 | 7.4 ± 1.7 |
Homozygote | 0.031 ± 0.001 | 8.0 ± 0.3 | 25.5 ± 0.9 | 29.5 ± 0.8 | 40.6 ± 3.5 | 199.0 ± 54.4 | 53.0 ± 1.3 | 7.9 ± 1.2 |
ca1ca2 | ||||||||
Wild type | 0.029 ± 0.001 | 8.5 ± 0.3 | 23.1 ± 1.7 | 25.0 ± 2.2 | 37.3 ± 0.9 | 195.8 ± 34.4 | 48.1 ± 1.0 | 7.7 ± 0.7 |
Heterozygote | 0.024 ± 0.001 | 7.3 ± 0.3 | 21.6 ± 0.9 | 26.3 ± 1.4 | 38.1 ± 1.0 | 180.9 ± 27.4 | 48.2 ± 0.7 | 7.6 ± 0.2 |
Homozygote | 0.029 ± 0.001 | 6.2 ± 0.2 | 21.2 ± 0.7 | 28.7 ± 0.9 | 37.4 ± 1.0 | 220.7 ± 15.9 | 53.3 ± 2.1 | 9.9 ± 0.7 |
ANOVAsb | ||||||||
Genotype | ns | WTa, HZab, HMb | ns | ns | ns | ns | ns | ns |
Line | ns | m1a, m2a, ca1ca2b | m1ab, m2a, ca1ca2b | ns | ns | ns | ns | ns |
Interaction | ns | ns | ns | ns | ns | ns | ns | ns |