Figure 3.

A, Levels of nitrite and conjugate produced by GST-U25 at pH 6.5 and 9.5 after 1 and 3 h. B and C, Activities of GST-U24 (B) and GST-U25 (C) with increasing pH after 1 h of incubation. All TNT conjugation reactions were performed at 20°C in 100 mm phosphate buffer with 150 μg of GST, 200 μm TNT, and 5 mm GSH in a total volume of 250 μL. Conjugation products and nitrite were measured by HPLC and Griess assays, respectively. Results are means of three biological replicates ± sd. [See online article for color version of this figure.]