PFT1/MED25 regulates root system architecture in Arabidopsis. Wild-type (Col-0), pft1-2, gPFT1, 35S:PFT1, med8, and pft1 med8 seeds were germinated and seedlings were grown for 8 d on agar-solidified 0.2× MS medium. A, Primary root length. B, Lateral root number. C, Lateral root length. Root traits were scored as indicated in “Materials and Methods.” Error bars represent se from 30 seedlings. Different letters indicate statistical differences at P < 0.05. D, Photographs of representative wild-type (Col-0), pft1-2, gPFT1, 35S:PFT1, med8, and pft1 med8 seedlings. Note that an opposite response is seen in lateral root formation and lengths between the pft1-2 mutant and PFT1-overexpressing seedlings. The experiment was repeated twice with similar results. Bar = 1 cm. [See online article for color version of this figure.]