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. 2014 Apr 30;165(2):880–894. doi: 10.1104/pp.114.239806

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Figure 5. — Effects of IAA on auxin-responsive reporter gene expression in wild-type, pft1-2, and 35S:PFT1 seedlings. Wild-type, pft1-2, and 35S:PFT1 seeds harboring the DR5:uidA gene construct were germinated and seedlings were grown for 7 d in agar-solidified 0.2× MS medium and then transferred for 8 h to liquid medium supplemented with or without IAA. Photographs show representative individuals of at least 15 GUS-stained seedlings analyzed. Note that pft1-2 and 35S:PFT1 seedlings show stronger and weaker GUS activity, respectively, than wild-type seedlings. Bars = 200 µm. [See online article for color version of this figure.]