PIN1 expression in wild-type, pft1-2, and 35S:PFT1 seedlings. PIN1::PIN1::GFP, pft1-2/PIN1::PIN1::GFP, and 35S:PFT1/PIN1::PIN1::GFP seeds were germinated and seedlings were grown on agar-solidified 0.2× MS medium. Seven days after germination, the seedlings were stained with propidium iodide and analyzed by confocal microscopy. A to C, Primary root apical meristems. D to F, Stage V LRP. G to I, Stage VII LRP. Representative photographs of primary roots and LRP are shown (n = 10). Note the increase and decrease of PIN1::PIN1::GFP in pft1-2 and 35S:PFT1 backgrounds, respectively. Bars = 100 μm. [See online article for color version of this figure.]