Software implementation |
Image acquisition |
Built directly into the pipeline with skeleton preview |
NS |
Skeletonization |
Based on exact morphological erosions of a shape |
Axes connected with straight-line segments |
Skeletonization |
Potential for future software releases to target differently shaped image objects |
Generates skeletons for branching structures only |
Axis definition |
Internode/awn filtering built directly into skeleton calculation with adjustable parameters |
Internodes and awns must be manually edited using a GUI
|
Axis definition |
User divides the primary axis three times |
User divides the primary axis two times |
Axis definition |
Skeleton points are selected on top of a _skel.jpg image, generating an accessory file |
Points selected within a skeleton graph and require manual editing of the skeleton |
Software execution |
Linear pipeline controlled with scripts |
Self-contained GUI
|
Compatibility |
Linux |
Windows, Mac OSX, and Linux |
Beta testing |
Image number |
More than 2,600 images in this study |
26 images |
Genetic mapping |
QTL mapping using 154 RILs in rice |
NS |
Phenotyping differences |
Primary axis internodes |
Length of individual rachis internodes |
Mean internode length only |
Primary axis thickness |
Mean and maximum thickness measures |
Mean thickness only |
Branches per internode |
Calculated at every internode (mean and maximum) |
NS |
Exsertion phenotypes |
Length and thickness |
NS |
Secondary/tertiary axes |
NS |
Branch length, count, and number of internodes |
Spikelet traits |
NS |
Count, length, and shape |