Table I. Summary statistics for DNA variant analysis at Rhg1 from whole-genome sequencing shows higher rates of polymorphism in SCN-resistant lines.

Copy No., Rhg1 copy number estimated from sequencing read depth or from fiber-FISH (asterisks). Variant Class, Whole-genome sequencing for 30 soybean lines and one G. soja line was analyzed for DNA variants and classified as SNPs, insertion, or deletion relative to the Williams 82 reference genome. The total number of DNA variants of each type across the 31-kb Rhg1 sequence are reported. Total, Sum of the SNP, insertion, and deletion variants. Variant Location columns report numbers of variants in each type of genome region. UTR, Untranscribed region.

Genotype	Variant Class					Variant Location
	Copy No.	SNPs	Insertion	Deletion	Total	Exon	Intron	Intergenic	UTR
LD00-3309	10.03	190	36	32	258	6	24	220	8
LG05-4292	9.23	195	38	34	267	6	25	228	8
4J105-34	9.53	196	35	32	263	6	24	225	8
CL0J095-46	9.76	195	36	32	263	6	24	225	8
LD02-4485	9.79	189	36	33	258	6	25	219	8
LD02-9050	9.56	177	32	28	237	6	24	199	8
Maverick	9.04	187	33	31	251	6	24	213	8
Cloud	7*	194	35	32	261	6	25	222	8
PI 209332	10*	197	35	33	265	6	27	224	8
PI 437654	3*	193	37	35	265	5	27	224	9
Peking	3*	200	38	33	271	6	25	232	8
PI 89772	3*	194	37	36	267	5	25	229	8
PI 90763	3*	192	37	34	263	5	25	224	9
LD01-5907	2.95	146	18	17	181	5	24	146	6
NE3001	0.99	63	3	6	72	4	23	42	3
LG05-4317	1.01	72	4	5	81	1	19	60	1
LG94-1128	1.02	45	2	3	50	1	15	33	1
LG92-1255	1.02	46	6	3	55	0	3	51	1
PI 518751	1.07	44	2	3	49	0	0	49	0
LG94-1906	1.03	26	0	2	28	0	0	28	0
LG03-3191	0.98	28	0	0	28	0	0	28	0
CL0J173-68	1.04	0	0	0	0	0	0	0	0
HS6-3976	1.02	0	0	0	0	0	0	0	0
LG04-4717	1.10	0	0	0	0	0	0	0	0
PI 398881	1.09	0	0	0	0	0	0	0	0
PI 427136	1.09	0	0	0	0	0	0	0	0
PI 507681B	1.00	0	0	0	0	0	0	0	0
LG90-2550	1.05	0	0	0	0	0	0	0	0
Prohio	0.97	0	0	0	0	0	0	0	0
LG98-1605	1.09	0	0	0	0	0	0	0	0
G. soja	1.10	42	3	4	49	1	3	45	0