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ER stress evidence was observed in fl4. A, Ultrastructure of developing endosperms of the wild type (WT) and fl4 (20 DAP). Normal ER configuration in the wild type (left, red arrowhead). Dilated ER in fl4 (right, red arrowhead). Bars = 500 nm. B, Immunoblot comparing BIP accumulation in wild-type and fl4 mature kernels. Anti-Tubulin was used as a sample loading control.
