Table I. Summary of AED proteins identified by 2D gel or ICPL analyses with their ICPL quantitation and significance as well as possible biological functions (Gene Ontology analysis; www.arabidopsis.org)ND, Not determined.
Gene | Locus | Protein (Predicted Size) | ICPL Ratio, Wild Type to eds1-2 | P (ICPL)a | Biological Process |
---|---|---|---|---|---|
AED1 | At5g10760 | Eukaryotic aspartyl protease family protein (49.4 kD) | ND | ND | Response to stress/response to abiotic or biotic stimulus/protein metabolism/signal transduction/other cellular processes |
ASPG1, AED2 | At3g18490 | Aspartic protease in guard cell1 (53.2 kD) | 1.39 | 0.20 | Response to stress/response to abiotic or biotic stimulus/protein metabolism |
AED3 | At1g09750 | Eukaryotic aspartyl protease family protein (47.6 kD) | 1.22 | 0.45 | Protein metabolism |
AED4 | At1g29660 | GDSL-motif lipase (40.1 kD) | 1.45 | 0.15 | Response to stress/response to abiotic or biotic stimulus/transport |
AED5 | At3g05180 | GDSL-motif lipase (42.3 kD) | ND | ND | Other metabolic processes |
XYL4, AED7 | At5g64570 | β-Xylosidase (84.3 kD) | 1.15 | 0.61 | Other metabolic and cellular processes |
PR2 | At3g57260 | Pathogenesis-related protein2 (37.3 kD) | 5.62 | 1.5E-11 | Response to stress/response to abiotic or biotic stimulus/signal transduction/other cellular and metabolic processes |
PR5 | At1g75040 | Pathogenesis-related protein5 (25.2 kD) | 2.9 | 3.2E-05 | Response to stress/response to abiotic or biotic stimulus/signal transduction/other cellular and metabolic processes |
LLP1, AED9 | At5g03350 | Legume lectin family protein (30.1 kD) | 2.0 | 0.007 | Unknown |
PNP-A, AED14 | At2g18660 | Plant natriuretic peptide A (14.5 kD) | 2.65 | 0.0001 | Response to stress/response to abiotic or biotic stimulus/electron transport or energy pathways/other metabolic and cellular processes |
AED15 | At2g43570 | Putative chitinase (29.7 kD) | 2.34 | 0.0009 | Other metabolic and cellular processes |
EP1, AED19 | At4g23170 | Cys-rich receptor-like kinase (29.7 kD) | 1.96 | 0.009 | Other cellular processes |
According to the Perseus statistical tool (www.perseus-framework.org; Cox and Mann, 2011).