Figure 2.

Absolute quantification of ICHO/ICOOH derivative pool sizes and camalexin in rosette leaves of nontreated (gray bars) and AgNO₃-treated (24 h after treatment; black bars) Col-0 plants. Rosette leaves of 10 nontreated/treated plants, cultivated in two independent experiments, were individually pooled and extracted. After treatment with a β-glucosidase, ICHO/ICOOH derivatives were quantified by UPLC/ESI(+)-QTOFMS. 5-HO-ICOOH and 6-HO-ICHO were below the detection limit (less than 0.2 and less than 0.1 nmol g⁻¹ fresh weight [FW], respectively). Error bars indicate sd (n = 10); n.d., not detected.