Figure 2. Activation of recipient CD8 T cells by MHC class I-deficient allogeneic BM.

B10.Q mice were conditioned with 3 Gy TBI on Day −1, anti-NK1.1 mAb on Days −7 and −2, and anti-CD154 mAb on Day 0. Conditioned mice received 20×10⁶ K^bD^b−/− TCD BM cells on Day 0. Mice in the control group were conditioned but did not receive BM cells. On Day 11 post-transplant, the expression of CD25, CD69, CD44 and CD62L on recipient CD8 T cells in the spleen and bone marrow was determined. (A) Cumulative data showing the percentages of CD25⁺, CD69⁺ and CD44^highCD62L^low CD8 T cells among total recipient CD8 T cells in the spleen and bone marrow. (B) Cumulative data showing the absolute numbers of CD25⁺, CD69⁺ and CD44^highCD62L^low recipient CD8 T cells in the spleen and bone marrow. Data are presented as mean ± SEM. *p<0.05 with t-test, for B10.Q recipients receiving K^bD^b−/− TCD BM cells versus those not receiving BM cells. p values of other groups are also shown. Each group contains 3 animals. Open bar: conditioned recipient without transplantation of K^bD^b−/− TCD BMCs. Black bar: conditioned recipient without transplantation of K^bD^b−/− TCD BMCs. SP: spleen. BM: bone marrow.