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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Am J Transplant. 2013 Dec 4;14(1):49–58. doi: 10.1111/ajt.12525

Figure 3. Specificity of rejection of MHC class I-deficient allogeneic BM by recipient CD8 T cells.

Figure 3

B10.S mice were conditioned with 3 Gy TBI on Day −1, anti-NK1.1 mAb on Day −1 and anti-CD154 mAb on Day 0, with or without CD4 depleting mAb administration on Day −1. After the conditioning, mice received 20×106 KbDb−/− B6 TCD BM cells. The percentage of donor B cell chimerism is shown in recipients of class I-deficient BMT over time in blood (A) and bone marrow (B). Day 4 and Day 11, one experiment is shown with 2–3 animals per group. Two experiments are shown for Day 7 with 6 animals in total. Each symbol represents an individual animal. Lines indicate mean values. No differences were detected between the groups when analyzed by a Kruskal-Wallis test. (C) and (D) B10.S mice were conditioned as above and did or did not receive 20×106 KbDb−/− B6 TCD BM cells. Total numbers (mean ± SD) of recipient (KH49+) BM cells from two tibias per animal are shown in C and mean WBC counts are shown in D. For Day 4 and Day 11, one experiment is shown with 2–3 animals per group. Two experiments are shown for Day 7 with 6 animals per group in total (Mean, ± SD).