Figure 7. Cytotoxicity of recipient CD8 T cells against MHC class I-deficient allogeneic BM.

B10.S mice were conditioned with 3 Gy TBI and anti-CD4 mAb on Day −1, anti-NK1.1 mAb on Days −7 and −2, and anti-CD154 mAb on Day 0 with or without (control) 20×10⁶ K^bD^b−/− TCD BM cells on Day 0. On Day 8–9 post-transplant, 2–3 recipients in each group were euthanized and their splenocytes were pooled. Splenic CD8 T cells were then isolated by anti-CD8 microbeads. These CD8 T cells were cocultured with Cr⁵¹-labeled K^bD^b−/− and syngeneic B10.S ConA-blasts for 4 hours. Levels of Cr⁵¹ in the supernatant were then determined and percent specific lysis was calculated as described (20). Data from two experiments performed with similar results are shown.