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. 2014 May 27;5(3):e01041-14. doi: 10.1128/mBio.01041-14

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Copyright © 2014 Kuehl et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 4 — Dde_3007 is required for methionine biosynthesis in D. alaskensis G20. (A) Comparison of gene fitness for 2,379 genes in LS4D minimal medium (x axis) versus LS4D minimal medium supplemented with 0.2% (wt/vol) Casamino Acids. Genes putatively involved in methionine (blue) and amino acid biosynthesis (red) are marked. Putative amino acid biosynthesis genes were identified using TIGRfam subroles (65). (B) Same as panel A for LS4D minimal medium (x axis) versus LS4D minimal medium supplemented with 1 µM methionine. (C) Growth on minimal LS4D medium of wild-type D. alaskensis G20 (top), JK00771 (Dde_3007 transposon mutant, right), JK00771 with pJK2 (Dde_3007, bottom), JK00771 with pMO9075 (empty vector, left). (D) Same as panel C with LS4D plus homocysteine medium. (E) Predicted pathway of methionine biosynthesis in D. alaskensis G20. Unknown enzymes are marked in red.