Fig. 3.

Light microscopy of GFP-C1 and mCherry-H2B in 70 nm resin sections using a widefield epifluorescence light microscope. (A) Overview of a 70 nm thick section through cells embedded in HM20, collected on a finder grid and imaged in phosphate buffered glycerol. A wide variety in expression level of both constructs can be seen. (B) Enlarged detail of an individual cell boxed in (A) showing mCherry-H2B within the nucleus, and GFP-C1 associated with the nuclear envelope and structures within the cytoplasm. (C, D) As above, for cells embedded in K4M. (E, F) As above, for cells embedded in LR White. White arrows indicate nucleoplasmic reticulum. Scale bars—A, C, E: 50 μm, B, D, F: 10 μm.