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. 2014 Aug;143(100):3–14. doi: 10.1016/j.ultramic.2014.02.001

Fig. 6.

Fig. 6

GFP in IRF sections is stable and active at atmospheric pressure and in vacuum. (A) Sequential images depicting the effect of vacuum pressure on the fluorescent signal recorded from a 200 nm IRF section, beginning at atmospheric pressure (Atm), proceeding stepwise to high vacuum, and returning to atmospheric pressure. (B) Overlaid fluorescent signal for GFP-C1, recorded at 200 Pa, onto the matching scanning electron micrograph recorded in high vacuum. Scale bars—25 μm.