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. Author manuscript; available in PMC: 2015 Apr 24.
Published in final edited form as: Cell. 2014 Apr 24;157(3):714–725. doi: 10.1016/j.cell.2014.04.005

Figure 3. Wanderlust uncovers rare B cell progenitors prior to the expression of CD10 or CD19.

Figure 3

A) Wanderlust trace showing the expression of CD24, CD34, CD38, and TdT (upper left panel). Density plots of four distinct TdT and CD24 populations defined within the CD34+CD38+ fraction (right). A histogram overlay of the Wanderlust values for cells contained in population I-V (dark blue boxes). The median Wanderlust values are indicated. B) Expression of the surrogate light chain of the pre-B cell receptor, λ5 and VpreB (top), and CD10 and IgH (i)ntracellular (bottom) across populations I though V. ‘*” denotes the uniform expression of IgHi in population V. C) Relative to population II, the amount of (C) IgH DJ and (D) IgH V(D)J rearrangement by qPCR of genomic DNA from prospectively isolated cells from populations II-V. Triplicate analysis of two biological replicates. Error bars are standard deviation. ‘*’ denotes uniform IgHi expression implying complete IgH V(D)J rearrangement. E) Pie charts summarizing the cellular contribution of human BMMNCs. The purple pie denotes CD34+CD38+ cells. Percentages are relative to all BMMNCs. See also Figure S3 for supporting data.