Table 2. mRNA content in visceral adipose tissue, liver and skeletal muscle.
Tumor necrosis factor (TNF)α, interleukin (IL)-6 and F4/80 mRNA in perigonadal visceral adipose tissue (V-AT), liver and skeletal muscle (SkM) from 3 months old (mo) untrained chow mice (UT-C), 15 month old untrained chow mice (UT-C), 15 month old untrained resveratrol (RSV) supplemented mice (UT-R), 15 month old exercise trained (T-C) and 15 month old exercise trained and RSV supplemented (T-R) whole body PGC-1α knockout (KO) and littermate wild type (WT) mice. Values are presented as means ± S.E.; n=8–10. Two-way ANOVA and one-way ANOVA tests were applied on logarithmically transformed data when appropriate. Mann-Whitney U nonparametric test was applied when appropriate
| 3 mo UT-C | 15 mo UT-C | 15 mo UT-R | 15 mo T-C | 15 mo T-R | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| gene | WT | KO | WT | KO | WT | KO | WT | KO | WT | KO | |
| V-AT | TNFα | 0.5 ± 0.1 | 0.8 ± 0.2 | 2.3 ± 0.7† | 0.7 ± 0.2# | 0.9 ± 0.2 | 1.3 ± 0.3* | 1.0 ± 0.3 | 0.7 ± 0.2 | 0.9 ± 0.1 | 1.4 ± 0.4 |
| IL-6 | 0.8 ± 0.2 | 0.6 ± 0.1 | 0.6 ± 0.1 | 0.4 ± 0.1 | 0.9 ± 0.1* | 0.8 ± 0.1* | 1.2 ± 0.1* | 0.7 ± 0.2# | 0.7 ± 0.1 | 0.5 ± 0.1 | |
| F4/80 | 0.8 ± 0.2 | 0.5 ± 0.2 | 1.2 ± 0.3 | 0.7 ± 0.3 | 0.6 ± 0.3 | 0.7 ± 0.1 | 1.3 ± 0.2 | 0.7 ± 0.3 | 0.7 ± 0.2 | 0.9 ± 0.3 | |
| Liver | TNFα | 0.3 ± 0.03 | 0.3 ± 0.04 | 0.6 ± 0.1† | 0.3 ± 0.03# | 0.5 ± 0.1 | 0.5 ± 0.1* | 0.5 ± 0.1 | 0.4 ± 0.04 | 0.5 ± 0.04 | 0.5 ± 0.1* |
| IL-6 | 0.2 ± 0.02 | 0.2 ± 0.01 | 0.2 ± 0.01 | 0.2 ± 0.02 | 0.1 ± 0.01 | 0.2 ± 0.03 | 0.2 ± 0.02 | 0.2 ± 0.02 | 0.2 ± 0.02 | 0.2 ± 0.03 | |
| F4/80 | 0.6 ± 0.04 | 0.5 ± 0.1 | 0.7 ± 0.1 | 0.4 ± 0.1# | 0.5 ± 0.1 | 0.7 ± 0.1 | 0.6 ± 0.1 | 0.5 ± 0.1 | 0.5 ± 0.03 | 0.7 ± 0.1 | |
| SkM | TNFα | 1.1 ± 0.1 | 0.7 ± 0.1 | 1.4 ± 0.2 | 1.6 ± 0.3† | 1.2 ± 0.3 | 1.6 ± 0.2 | 2.6 ± 0.6* | 1.9 ± 0.2 | 1.9 ± 0.3 | 2.4 ± 0.4 |
| IL-6 | 0.7 ± 0.1 | 0.5 ± 0.1 | 1.0 ± 0.1(†) | 1.0 ± 0.1† | 0.7 ± 0.2 | 1.0 ± 0.1 | 0.7 ± 0.1* | 0.7 ± 0.1* | 0.9 ± 0.1* | 0.7 ± 0.1* | |
| F4/80 | 2.4 ± 0.3 | 2.3 ± 0.3 | 1.2 ± 0.2† | 1.6 ± 0.3 | 0.8 ± 0.2 | 1.6 ± 0.3# | 3.7 ± 1.1* | 3.0 ± 0.4* | 2.0 ± 0.3* | 3.5 ± 0.4#* | |
Significantly different from 3 month old UT-C within genotype, p<0.05.
Tends to be significantly different from 3 month old UT-C within genotype, 0.05≤p<0.1.
Significantly different from 15 month old UT-C within genotype, p<0.05.
Significantly different from WT within group, p<0.05.