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Comparison of a portion of 800 MHz, cryo-probe 1H NMR spectra of the same pooled human serum sample obtained by suppressing protein signals (a) by ultrafiltration using 3 kDa cutoff filter and (b) by protein precipitation using methanol (1:2). Note the peaks marked with asterisks are attenuated significantly in part a compared to part b. Of these, the identity for one metabolite (2-oxoisocaproate) was established in this study and the others are unidentified.
