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Siglec-F-dependent negative regulation of allergen-induced eosinophilia depends critically on the experimental model used. WT, siglec-FR114D and siglec-F−/− mice were subjected to allergic inflammation using either model 1 or model 2. Total and differential cells counts were enumerated in Diff Quik stained cytospins of collagenase digests of lung tissue (LMs; lymphomononuclear cells). Data are expressed as mean ± S.E.M., n = 4–6/group, *p ≤ 0.05 using Mann Whitney U Test.
