Fig. 4.

Comparable expression of siglec-F ligands in the lung tissue of model 1 and model 2 induced allergic airway inflammation. The expression of siglec-F ligands in lung tissue taken from PBS or OVA-treated WT mice was measured using pre-complexes of siglec-F-Fc/anti-Fc. Sections shown in lower panels were treated with sialidase prior to staining. Sections were analyzed by confocal microscopy (LSM 700 microscope and Zen 2009 software; Carl Zeiss) and images collected with an α-Plan-Apochromat 40× NA 1.46 objective. Scale bars represent 50 μm.