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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Exp Gerontol. 2013 Jun 24;48(9):947–959. doi: 10.1016/j.exger.2013.06.002

Fig. 3.

Fig. 3

Effect of age on electron flow in mitochondria isolated from mice heart: mitochondria were isolated from young (2–3 months) or old mice (22–28 months) heart as described in Materials and methods. (A) A representative OCR measurement figure from XF24 instrument using point-to-point data due to electron flow in the mitochondria with pyruvate and malate as substrates, and the effect of rotenone, succinate, antimycin A and Asc/TMPD as described in Materials and methods. (B) Young and old mouse mitochondria with state 3 µ, rotenone, succinate, antimycin A and Asc/TMPD mediated OCR (young n = 5, old n = 4). (C) Absolute pH levels during the electron flow; (D) absolute oxygen levels during the electron flow in the micro-chamber. *Significantly lower than young mice (ANOVA; p < 0.001).