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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Exp Gerontol. 2013 Jun 24;48(9):947–959. doi: 10.1016/j.exger.2013.06.002

Fig. 4.

Fig. 4

Effect of age on mitochondrial coupling in IFM mitochondria: IFM and SSM mitochondria were isolated as described in Materials and methods. (A) A representative coupling data of isolated IFM mitochondria in young and old mice in XF24 analyzer that show the effect of ADP, oligomycin, FCCP and antimycin A; (C) basal, state 3, state 40, state 3 µ and antimycin A respiration in IFM mitochondria from young or old mice (young n = 4, and old n = 4); (E) absolute pH levels in the micro-chamber due to coupling in IFM mitochondria; (B) a representative coupling experiment of isolated SSM mitochondria in young and old mice hearts and the effect of ADP, oligomycin, FCCP and antimycin A; (D) basal, state 3, state 40, state 3 µ and antimycin A respiration in SSM mitochondria from young or old mice (young n = 4, and old n = 4); (F) absolute pH levels in the micro-chamber due to coupling in SSM mitochondria. *Significantly lower than old SSM mitochondria (ANOVA; p < 0.01).