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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Exp Gerontol. 2013 Jun 24;48(9):947–959. doi: 10.1016/j.exger.2013.06.002

Fig. 5.

Fig. 5

Effect of age on mitochondrial electron flow in SSM and IFM mitochondria isolated from mice heart: SSM and IFM mitochondria were isolated as described in Materials and methods. (A) A representative figure of electron flow experiment of isolated SSM mitochondria in young (2–3 months old, n = 4) and old mice (22–28 months old, n = 3) in XF24 analyzer; (C) state 3 µ, rotenone, succinate, antimycin A and ASC/TMPD-mediated OCR in SSM mitochondria from young or old mice. (E) Absolute pH levels in the micro-chamber due to electron flow in SSM & IFM mitochondria. (B) A representative figure of electron flow of isolated IFM mitochondria in young and old mice heart. (D) State 3 µ, rotenone, succinate, antimycin A and ASC/TMPD-mediated OCR in IFM mitochondria from young or old mice. (F) Absolute oxygen levels in the micro-chamber due to electron flow in SSM & IFM mitochondria. *Significantly lower than young IFM (ANOVA; p < 0.0001).