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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Exp Gerontol. 2013 Jun 24;48(9):947–959. doi: 10.1016/j.exger.2013.06.002

Fig. 6.

Fig. 6

Mitochondrial coupling and electron flow in young and old Sod2+/− mice heart: Mitochondria were isolated from young (5–7 months old) or old (28 months old) Sod2+/− mice hearts as described in Materials and methods. (A) A representative coupling assay using isolated lung mitochondria and OCR response to ADP, Oligomycin, FCCP and antimycin A using point-to-point measurements in XF24. (C) Graph of basal (state 2), state 3, state 40, state 3 µ and antimycin A respiration in young and old Sod2+/− mice heart mitochondria (young n = 3, old n = 3). (E) Absolute pH levels in the coupling assay; (B) representative figure of electron flow assay using point-to-point data in the mitochondria using pyruvate and malate as substrates, and its modulation due to injections of rotenone, succinate, antimycin A and Asc/TMPD. (D) Young and old Sod2+/− mouse mitochondria with state 3 µ, succinate, antimycin A and Asc/TMPD mediated OCR; (F) absolute pH levels in the electron flow assay.