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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Clin Immunol. 2013 Mar 26;148(1):44–55. doi: 10.1016/j.clim.2013.03.008

Figure 2. ROS levels modestly contribute to XRT-induced phosphorylation of NEMO.

Figure 2

(A) Ratio of GSSG to GSH+GSSG (total GSH) in U937 cells 1-16 hrs after irradiation with 0, 2, and 20 Gy. Data are shown as the average ratio +/− standard error from three independent experiments. (B) Phosphorylation of NEMO at 1-16 hrs after (+) pre-treatment with 3.95μM BSO for 3 hrs prior to 0, 2, or 20 Gy irradiation or (-) radiation alone in U937 mutant cells (C) Phosphorylation of NEMO at 1-16 hrs after pre-treatment with 10mM NAC for 3 hrs prior to 0, 2, or 20 Gy irradiation in U937 mutant cells. (A-C) *(p=<0.05), **(p=<0.01), ***(p=<0.001); and NS, not significant. For all experiments, data is shown as normalized average MFI± S.E.M from three independent experiments unless otherwise specified.