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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Clin Immunol. 2013 Mar 26;148(1):44–55. doi: 10.1016/j.clim.2013.03.008

Figure 3. Phosphorylation of NEMO after XRT and Doxorubicin requires ATM.

Figure 3

(A) U937 cells treated with 10μM Ku55933 (+) versus no inhibitor (-) prior to irradiation (0, 2, or 20 Gy) at 1-24 hrs. (B) Western blot analysis of NEMO and ATM phosphorylation in U937 and ATM deficient GM0536 cells. β-actin served as a loading control. (C) U937 cells treated with 10μM Ku55933 (+) versus no inhibitor (-) prior to 0.25μg/ml Doxorubcin at 1-24 hrs. (D)

Phosphorylation of NEMO and ATM were measured by western blot analysis in untransfected U937 and U937+negative control siRNA cells. NEMO and ATM were phosphorylated after treatment with 20 Gy but not in untreated ( 0 Gy) U937 cells. Radiation did not phosphorylate ATM or NEMO in ATM knocked down cells. β-actin served as a protein loading control. *(p=<0.05), **(p=<0.01), ***(p=<0.001); and NS: not significant.