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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Clin Immunol. 2013 Mar 26;148(1):44–55. doi: 10.1016/j.clim.2013.03.008

Figure 6. XRT-induced maturation and responsiveness of U937 cells requires NFκB.

Figure 6

(A) Inhibition of CD40, CD86, and MHC Class II phosphorylation at 48 hrs by NBD peptide (+) versus no peptide (-) after 2 or 20Gy XRT compared to CD40, CD86, and MHC Class II phosphorylation after XRT alone. NS=not significant, **(p=<0.01), and ***(p=<0.001). (B) T cell proliferation was measured following combination of CD4+ T cells with irradiated (2 or 20 Gy) U937 cells and U937 pretreated with non-specific control peptide increased T cell proliferation. Irradiated U937 cells pre-treated with NBD peptide showed reduced T cell proliferation. Responder cells alone (purified CD4+ T cells), stimulator cells alone (irradiated U937 cells), and CD4+T cells were combined with unirradiated U937 cells and served as negative controls. Shown is the average absorbance (from triplicates)±S.E.M. from three independent experiments. *(p=0.05) and **(p=<0.01).